E. coli FABI Antibody


Catalog #: ABCESPA1327
Product Features
Immunogen: Recombinant E.coli Enoyl-ACP Reductase / FABI protein 
Clonality:  Rabbit PAb 
Isotype: Rabbit IgG
Buffer: 0.2 μm filtered solution in PBS with 5% trehalose
Reactivity: E. coli
Specificity: E.coli Enoyl-ACP Reductase / FABI
Application: ELISA
Recommend dilution:  ELISA: 0.5-1 μg/mL. This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect E.Coli FABI. The detection limit for E.Coli FABI is approximately 0.00975 ng/well.
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background: Enoyl-ACP reductase, also known as  NADH-dependent enoyl-ACP reductase and FABI, is a cell inner membrane and  peripheral membrane protein which belongs to the short-chain  dehydrogenases/reductases (SDR) family and FabI subfamily. Microorganisms produce  many kinds of antibiotics which function in an antagonistic capacity in nature  where they have much competition. Bacterial FAS provides essential fatty acids  for use in the assembly of key cellular components. Among them, FABI is an  enoyl-ACP reductase which catalyzes the final and rate-limiting step of  bacterial FAS. The antibiotic diazaborine interferes with the activity by  binding to the protein. FABI is a potential target for selective antibacterial  action, because it shows low overall sequence homology with mammalian enzymes.  Various compounds have been reported as inhibitors of bacterial FabI-inhibitory  compounds.

, ,