E. coli STX2B Antibody

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Catalog #: ABCESPA1330
Product Features
Immunogen: Recombinant Enterohemorrhagic E. coli 
Clonality:  Mouse MAb
Cloneno: 13
Isotype: Mouse IgG1
Buffer: 0.2 μm filtered solution in PBS with 5% trehalose
Reactivity: E. coli
Specificity: Enterohemorrhagic E. coli (EHEC) STX2BNo cross-reactivity in ELISA withE.coli cell lysate
Application: ELISA
Recommend dilution:  ELISA: 0.5-1 μg/mL. This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Enterohemorrhagic E. coli (EHEC) STX2B. The detection limit for Enterohemorrhagic E. coli (EHEC) STX2B is approximately 0.039 ng/well.
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background: E. Coli STX2B is a subunit of Stx2. Stx2, together with Stx1, formed a family of related toxins which are known as shiga toxins. Shiga toxins are mainly produced by the bacteria S. dysenteriae and the Shigatoxigenic group of Escherichia coli, which includes serotypes O157:H7, O104:H4, and other enterohemorrhagic E. coli (EHEC). A total of 3222 outbreak cases (including 39 deaths) have been reported in northern Germany in May through June 2011. The outbreak strain was typed as an enteroaggregative Shiga-toxin–producing E. coli O104:H4, producing extended-spectrum beta-lactamase. The toxin has two subunits—A and B. E. Coli STX2B is the B subunit. It is a pentamer that binds to specific glycolipids on the host cell, specifically globotriaosylceramide. Following this, the A subunit is internalised and cleaved into two parts. Stx2 has been found to be approximately 400 times more toxic (as quantified by LD50 in mice) than Stx-1. The Stx1 and Stx2 B subunits form a pentameric structure that binds to globotriaosylceramidereceptors on eukaryotic cells and promotes endocytosis
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