| Product Features | |
|---|---|
| Immunogen: | Recombinant Ferret CD4 protein |
| Clonality: | Mouse MAb |
| Cloneno: | 2 |
| Isotype: | Mouse IgG1 |
| Buffer: | 0.2 μm filtered solution in PBS with 5% trehalose |
| Reactivity: | Ferret |
| Specificity: | Ferret CD4No cross-reactivity in ELISA withHuman CD4Mouse CD4 |
| Application: | ELISA, FCM |
| Recommend dilution: | ELISA: 0.5-1 μg/mL. This antibody can be used at 0.5-1 μg/ml with the appropriate secondary reagents to detect Ferret CD4. FCM: 0.5-2 μg/Test |
| Storage: | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
| Background: | T-cell surface glycoprotein CD4, is a single-pass type I membrane protein. CD4 contains three Ig-like C2-type (immunoglobulin-like) domains and one Ig-like V-type (immunoglobulin-like) domain. CD4 is a glycoprotein expressed on the surface of T helper cells, regulatory T cells, monocytes, macrophages, and dendritic cells. The CD4 surface determinant, previously associated as a phenotypic marker for helper/inducer subsets of T lymphocytes, has now been critically identified as the binding/entry protein for human immunodeficiency viruses (HIV). The human CD4 molecule is readily detectable on monocytes, T lymphocytes, and brain tissues. All human tissue sources of CD4 bind radiolabeled gp120 to the same relative degree; however, the murine homologous protein, L3T4, does not bind the HIV envelope protein. CD4 is a co-receptor that assists the T cell receptor (TCR) to activate its T cell following an interaction with an antigen presenting cell. Using its portion that resides inside the T cell, CD4 amplifies the signal generated by the TCR. CD4 interacts directly with MHC class II molecules on the surface of the antigen presenting cell via its extracellular domain. The CD4 molecule is currently the object of intense interest and investigation both because of its role in normal T-cell function, and because of its role in HIV infection. CD4 is a primary receptor used by HIV-1 to gain entry into host T cells. HIV infection leads to a progressive reduction of the number of T cells possessing CD4 receptors. |
Ferret CD4 Antibody
$322.00 – $847.00