Listeria monocytogenes FLAA Antibody


Catalog #: ABCESPA1336
Product Features
Immunogen: Recombinant Listeria monocytogenes Listeria monocytogenes flagellin / FlaA protein 
Clonality:  Rabbit MAb
Cloneno: 121
Isotype: Rabbit IgG
Buffer: 0.2 μm filtered solution in PBS with 5% trehalose
Reactivity: Listeria monocytogenes
Specificity: Listeria monocytogenes Listeria monocytogenes flagellin / FlaA
Application: ELISA
Recommend dilution:  ELISA: 0.1-0.2 μg/mL. This antibody can be used at 0.1-0.2 μg/mL with the appropriate secondary reagents to detect Listeria monocytogenes Listeria monocytogenes flagellin / FlaA. The detection limit for Listeria monocytogenes Listeria monocytogenes flagellin / FlaA is approximately 0.0195 ng/well.
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background: The role of flagella and motility in  the attachment of the foodborne pathogen Listeria monocytogenes to various surfaces is mixed with some systems  requiring flagella for an interaction and others needing only motility for  cells to get to the surface. In nature this bacterium is a saprophyte and  contaminated produce is an avenue for infection. Previous studies have  documented the ability of this organism to attach to and colonize plant tissue.  Motility mutants were generated in three wild type strains of L. monocytogenes by deleting either FlaA, the gene encoding flagellin,  or motAB, genes encoding part of the flagellar motor, and tested for both the  ability to colonize sprouts and for the fitness of that colonization. The motAB mutants were not affected in the colonization of  alfalfa, radish, and broccoli sprouts; however, some of the FlaA mutants showed reduced colonization ability. The best  colonizing wild type strain was reduced in colonization on all three sprout  types as a result of a FlaA deletion. A mutant in another  background was only affected on alfalfa. The third, a poor alfalfa colonizer  was not affected in colonization ability by any of the deletions. Fitness of  colonization was measured in experiments of competition between mixtures of  mutant and parent strains on sprouts. Here the FlaA  and motAB mutants of the three strain  backgrounds were impaired in fitness of colonization of alfalfa and radish  sprouts, and one strain background showed reduced fitness of both mutant types  on broccoli sprouts. Together these data indicate a role for flagella for some  strains to physically colonize some plants, while the fitness of that  colonization is positively affected by motility in almost all cases.

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