Ni-NTA (His-tag Affinity) Magnetic Beads


Ni-NTA (His-tag Affinity) Magnetic Beads are nano-superparamagnetic beads coupled with nickel-charged nitrilotriacetic acid (Ni-NTA). With a fast magnetic response rate, high protein binding capacity and low non-specific binding, Ni-NTA (His-tag Affinity) Magnetic Beads provide a rapid and efficient method to purify His-tagged fusion proteins from crude cell lysates. The beads are simply added to the crude cell lysate and His-tagged fusion proteins will bind to the beads. After washing the unbound proteins off, the target His-fusion proteins can be eluted for downstream experiments. The process can be completed manually or fully automated for high throughput applications. NTA has four chelation sites for nickel ions and thus binds nickel more tightly than other metal-chelating purification systems that only have three sites available for interaction with metal ions. The extra chelation site prevents nickel ion leaching and results in a greater binding capacity and higher purity of the extracted protein than those obtained using other metal-chelating purification systems.

SKU Pack Size PriceQuantity
MBPP-04000 25mg/1ml $135.00
MBPP-04001 50mg/1ml $248.00
MBPP-04002 200mg/4ml $818.00
MBPP-04003 1g/20ml $3,224.00
Simple and Efficient Extraction Procedure

Simple and Efficient Extraction Procedure

Product Features
Specificity: Purification of His-tagged fusion proteins
Magnetic Separation: Fast response, complete purification process within 30min, no chromatograph, no centrifugation, maximally preserve protein activity
Excellent Suspension Ability: Nanometer scale, rapid conversion from solid-phase to solution and vice versa
High Binding Capacity: Efficient coupling, higher binding surface area
Low non-specific Binding: Hydrophilic microspheres, low non-specific binding
Compatibility: Fully compatible with automation and LC-MS/MS analyses
Product Specifications
Concentration: 50mg/ml
Diameter: 500nm
pH stability: 3-12
30min sedimentation rate: <0.1%
Magnetic response rate: >30emu/g
Solvent: 1mM NiSO4, 50mM Tris-HCl (pH 8.0), containing 20% ethanol
Binding capacity: 10-60µg His-tagged fusion protein per mg magnetic beads
Database and Results

Database and Results

Ni-NTA (His-tag Affinity) Magnetic Beads is stored at 2-8°C, 2 year.
Catalog No.
MBPP-04001 ABA-04001 (Avanbio)
MBPP-04002 ABA-04002 (Avanbio)
MBPP-04003 ABA-04003 (Avanbio)
Pack Size

25mg/1ml, 50mg/1ml, 200mg/4ml, 1g/20ml

Ni-NTA (His-tag Affinity) Magnetic Beads

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