Schistosoma japonicum GST Antibody


Catalog #: ABCESPA1249
Product Features
Immunogen: Recombinant GST protein
Clonality:  Mouse MAb
Cloneno: 6C10G4
Isotype: Mouse IgG1
Buffer: 0.2 μm filtered solution in PBS with 5% trehalose
Reactivity: Schistosoma japonicum
Specificity: Recognize GST Tag in fusion proteins.No cross-reactivity with E.coli cell lysate in ELISA.
Application: WB
Recommend dilution:  WB: 1/1000-1/10000
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background: Genetic engineers have used  glutathione S-transferase to create the GST gene fusion  system. This system is used to purify and detect proteins of interest. In a GST  gene fusion system, the GST sequence is incorporated into an expression  vector alongside the gene sequence encoding the protein of interest.  Induction of protein expression from the vector’s promoter results in  expression of a fusion protein: the protein of interest fused to the GST  protein. This GST-fusion protein can then be purified from cells via its high  affinity for glutathione. GST is commonly used to create fusion proteins.  The tag has the size of 220 amino acids (roughly 26 KDa), which, compared to other tags like the myc- or the FLAG-tag, is quite big. However, many  commercially-available sources of GST-tagged plasmids include  a thrombin domain for cleavage of the GST tag during protein  purification.

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