Product Features | |
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Immunogen: | Recombinant Canine TNF-alpha Protein |
Clonality: | Mouse MAb |
Cloneno: | 1F8D10 |
Isotype: | Mouse IgG1 |
Buffer: | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Canine TNF-alpha (rCanine TNF-alpha; Catalog #70003-DNAE; NP_001003244.4; Val 77-Leu 233). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
Reactivity: | Canine |
Specificity: | Canine TNF-alpha No cross-reactivity in ELISA withHuman TNF-αMouse TNF-αRat TNF-αE.coli cell lysate |
Application: | ELISA |
Recommend dilution: | ELISA: 0.5-1 μg/mL. This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Canine TNFα. The detection limit for Canine TNFα is approximately 0.625 ng/well. |
Storage: | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
Background: | Tumor necrosis factor alpha (TNF-alpha), also known as TNF, TNFA or TNFSF2, is the prototypic cytokine of the TNF superfamily, and is a multifunctional molecule involved in the regulation of a wide spectrum of biological processes including cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. Two receptors, TNF-R1 (TNF receptor type 1; CD120a; p55/60) and TNF-R2 (TNF receptor type 2; CD120b; p75/80), bind to TNF-alpha. TNF-alpha protein is produced mainly by macrophages, and large amounts of this cytokine are released in response to lipopolysaccharide, other bacterial products, and Interleukin-1 (IL-1). TNF-alpha is involved in fighting against the tumorigenesis, thus, is regarded as a molecular insight in cancer treatment. |
Canine TNFa Antibody
$322.00 – $847.00