Aequorea victoria GFP Antibody


Catalog #: ABCESPA1324
Product Features
Immunogen: Recombinant Aequorea victoria GFP protein 
Clonality:  Mouse MAb
Cloneno: 5
Isotype: Mouse IgG1
Buffer: 0.2 μm filtered solution in PBS with 5% trehalose
Reactivity: Aequorea victoria
Specificity: Aequorea victoria GFPNo cross-reactivity in ELISA withE.coli cell lysate
Application: ELISA, IP, ICC/IF, IF
Recommend dilution:   ELISA: 0.5-1 μg/mL. This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Aequorea victoria GFP. The detection limit for Aequorea victoria GFP is approximately 0.16 ng/well.      ICC/IF: 10-25 μg/mL      IP: 1-4 μg/mg of lysate
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background: The green fluorescent protein (GFP) is a protein that exhibit bright green fluorescence when exposed to blue light. GFPSparkTM is an improved variant of the green fluorescent protein GFP. It possesses bright green fluorescence (excitation/ emission max = 487 / 508 nm) that is visible earlier than fluorescence of other green fluorescent proteins. GFPSparkTM is mainly intended for applications where fast appearance of bright fluorescence is crucial. Its amazing ability to generate a highly visible, efficiently emitting internal fluorophore is both intrinsically fascinating and tremendously valuable. It is specially recommended for cell and organelle labeling and tracking the promoter activity.

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