| Product Features | |
|---|---|
| Immunogen: | Recombinant Rhesus HER2 / ErbB2 protein |
| Clonality: | Mouse MAb |
| Cloneno: | 10A7F7 |
| Isotype: | Mouse IgG1 |
| Buffer: | This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Rhesus HER2 / ErbB2 (Catalog#90020-K08H; XP_001090430.1; Met 1-Thr 652). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography. |
| Reactivity: | Rhesus |
| Specificity: | Rhesus HER2 / ErbB2Has cross-reactivity in ELISA withHuman ErbB2No cross-reactivity in ELISA withHuman EGFRHuman ERBB3Human ERBB4Human cell lysate (293 cell line) |
| Application: | ELISA |
| Recommend dilution: | ELISA: 0.5-1 μg/mL. This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect Rhesus ErbB2. The detection limit for Rhesus ErbB2 is approximately 0.0195 ng/well. |
| Storage: | This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -70℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles. |
| Background: | Human epidermal growth factor receptor 2 (HER2), also known as ErbB2, NEU, and CD340, is a type I membrane glycoprotein, and belongs to the epidermal growth factor (EGF) receptor family. HER2 protein cannot bind growth factors due to the lacking of ligand binding domain of its own and autoinhibited constitutively. However, HER2 forms a heterodimer with other ligand-bound EGF receptor family members, therefore stabilizes ligand binding and enhances kinase-mediated activation of downstream molecules. HER2 plays a key role in development, cell proliferation and differentiation. HER2 gene has been reported to associate with malignancy and a poor prognosis in numerous carcinomas, including breast, prostate, ovarian, lung cancers and so on. |
Rhesus ErbB2/HER2 Antibody
$322.00 – $847.00