RSV RSV-G Antibody


Catalog #: ABCESPA1243
Product Features
Immunogen: Recombinant Human RSV Glycoprotein G / RSV-G 
Clonality:  Mouse MAb
Cloneno: 9H2G5C6
Isotype: Mouse IgG1
Buffer: 0.2 μm filtered solution in PBS with 5% trehalose
Reactivity: RSV
Specificity: Human RSV Glycoprotein G/RSV-GNo cross-reactivity with Human cell lysate (293 cell line) in ELISA
Application: WB, ELISA
Recommend dilution:  WB: 1-2 μg/mL      ELISA: 0.5-1 μg/mL. This antibody can be used at 0.5-1 μg/mL with the appropriate secondary reagents to detect HRSV glycoprotein G. The detection limit for HRSV glycoprotein G is approximately 0.039 ng/well.
Storage: This antibody can be stored at 2℃-8℃ for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20℃ to -80℃. Preservative-Free.Sodium azide is recommended to avoid contamination (final concentration 0.05%-0.1%). It is toxic to cells and should be disposed of properly. Avoid repeated freeze-thaw cycles.
Background: Human respiratory syncytial virus  (HRSV) is the most common etiological agent of acute lower respiratory tract  disease in infants and can cause repeated infections throughout life. It is  classified within the genus pneumovirus of the family paramyxoviridae. Like  other members of the family, HRSV has two major surface glycoproteins (G and F)  that play important roles in the initial stages of the infectious cycle. HRSV G  protein is a type II glycoprotein of 289-299 amino acids (depending on the  virus strain) with a signal/anchor hydrophobic domain and is extensively  modified by the addition of both N-and O-linked oligosaccharides to achieve the  mature form of 80-90 kDa. The C-terminal ectodomain of the G protein has a  central region and four cysteines which are conserved in all HRSV isolates and  have been proposed as the putative receptor binding site. The G protein  mediates attachment of the virus to the host cell membrane by interacting with  heparan sulfate, initiating the infection. As similar to mucins in amino acid  compositions, the RSV G protein can interact with host CX3CR1, the receptor for  the CX3C chemokine fractalkine, and thus modulates the immune response and  facilitate infection. Secreted glycoprotein G helps RSV escape  antibody-dependent restriction of replication by acting as an antigen decoy and  by modulating the activity of leukocytes bearing Fcgamma receptors. Unlike the  other paramyxovirus attachment proteins, HRSV-G lacks both neuraminidase and  hemagglutinating activities.

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